Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli.

The gene for gamma-glutamylcysteine synthetase from Thiobacillus ferrooxidans has low homology to its Escherichia coli equivalent and is linked to the gene for citrate synthase.

The gene for gamma-glutamylcysteine synthetase (gshA) from Thiobacillus ferrooxidans was isolated from a family of cosmids by its ability to complement an Escherichia coli gshA trxA double mutant which was unable to grow on minimal medium lacking glutathione. The predicted sequence of the gamma-glutamylcysteine synthetase was found to have only 18% amino acid sequence identity to the equivalent...

Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli

Alanyl - tRNA synthetase gene of the extreme acidophi I ic chemol ithoautotrophic Thiobacillus ferrooxidans is highly homologous to alas

Laboratoire de Chirnie Bact&ienne, lnstitut de Biologie Structurale et de Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille C dex 2of France The alas gene of Thiobacillus ferrooxidans has been cloned and sequenced and its expression in Escherichia coli and T. fermoxidans analysed. The same genOmiC Organization to that in Em COli (I'ed-recX-ahS) has been found in T. ferrooxidans. The r e ...

The structural gene for rusticyanin from Thiobacillus ferrooxidans: cloning and sequencing of the rusticyanin gene.

The rusticyanin gene from the acidophilic chemolithotroph Thiobacillus ferrooxidans has been cloned and sequenced. A central portion of the gene was identified by PCR reactions utilising primers optimised for codon bias followed by nested PCR with degenerate primers. The 5' and 3' ends of the rusticyanin gene were then cloned using degenerate primers to each end and anchor sequences to the know...

Cloning and optimization of phytase enzyme gene expression in Escherichia coli

Introduction Phytase is an enzyme that has the ability to break down phytic acid into myoinositol and mineral phosphate, and widely uses as an additive in animal foods. The aim of this study was to achieve a high level of bacterial phytase expression in PET26b expression host. Materials and Methods To generate the recombinant phytase enzyme, the target gene was introduced into the expression ...